TABLE 3.

Bacteria Real-time PCR primers.

Target	Primer sequence (5′-3′)	Anneal Tm (°C)
Bacteroides fragilis Lactobacillus	F-ATAGCCTTTCGAAAGRAAGAT R-ATAGCCTTTCGAAAGRAAGAT F-GCAGCAGTAGGGAATCTTCCA R-GCATTYCACCGCTACACATG	50    60
Bacillus coagulans	F-GGTGGTAAATACTACGGTAATGGGGT R-GTGTCTAAATTACTGGTTGATTCGT	58
Sulfate-reducing bacteria	F-TGGCAGATMATGATYMACGGG R-GGGCCGTAACCGTCCTTGAA	60
Akkermansia muciniphila	F-CCTTGCGGTTGGCTTCAGAT R-CAGCACGTGAAGGTGGGGAC	55
Bifidobacterium	F-GCCACATTGGGACTGAGATA R-CCTACGAGCCCTTTACGC	60
All bacteria	F-TCCTACGGGAGGCAGCAGT R-GGACTACCAGGGTATCTATCCTGTT	58