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. 2021 Mar 24;11:6751. doi: 10.1038/s41598-021-86147-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Growth, differentiation and secretion profiles of BMSCs and CBMSCs after priming with either standard osteogenic differentiation supplements, BMP-4, or chondrogenic supplements. Scatter plots represent all MSC donor samples pooled regardless of priming, while bar charts present Mean ± SEM of each priming condition. *Significant difference between MSC origin. (A) Cell proliferation after 6 days of priming as assessed by Pico Green Assay for DNA content. (B) Intracellular ALP expression after 6 days of priming. (C) Multiplex array analysis of soluble secreted osteogenic cytokines; Osteoprotegerin (OPG), Osteocalcin (OC), and Osteopontin (OPN), immunogenic cytokines; Interleukin 6 and 8 (IL-6,8), Monocyte chemoattractant protein 1 (MCP-1), as well as angiogenic factors; Vascular Endothelial Growth Factor (VEGF). Statistical analysis was by unpaired Student’s t-test for single experimental condition (dot plots) and by two-way ANOVA followed by Holm–Sidak’s multiple comparison test for multiple experimental conditions (histograms). *Significant differences between MSCs of different tissue origin.