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. 2021 Mar 24;11:6787. doi: 10.1038/s41598-021-86204-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TgFNTs are not required for tachyzoite proliferation in vitro. (a) Data from a single representative experiment showing the growth of the different parasite lines (wild-type, blue; Δtgfnt1, white; Δtgfnt2, grey; Δtgfnt3, red; Δtgfnt1/2/3, dark red). Parasite growth was normalized to the maximum level reached for each parasite line after subtraction of the background fluorescence. The mean and SD from triplicate measurements shown. Where not shown, error bars fall within the symbols. For clarity, only positive or negative error bars are shown for the data for certain parasite lines. (b) The maximum growth rate for each parasite line. The bars show the mean ± SD obtained from three independent experiments for each line, and the symbols show the results obtained in individual experiments. There was no significant difference in the maximum growth rate between any of the parasite lines (P = 0.8; one-way ANOVA).