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. 2021 Mar 24;11:6787. doi: 10.1038/s41598-021-86204-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Measurements of the effect of l-lactate on cytosolic pH (pH_cyt) reveal a role for TgFNT1 in lactate transport across the parasite plasma membrane. Extracellular tachyzoites were loaded with BCECF and suspended in Saline Solution at 4 °C. The grey arrows denote the addition of 10 mM l-lactate. The red arrow denotes the addition of the protonophore CCCP (10 µM). The top panels show data for Δtgfnt1 (b), Δtgfnt2 (c), Δtgfnt3 (d) parasites and their wild-type parental parasites (a). The bottom panels show data for Δtgfnt1 parasites complemented with tgfnt1 (e), tgfnt2 (f) or tgfnt3 (g). The traces are from a single experiment for each parasite line, and are representative of those obtained in at least three independent experiments. The time taken to reach a minimum pH_cyt value after the addition of l-lactate was > 400 s in all experiments for Δtgfnt1 parasites complemented with tgfnt2, compared to < 150 s in all experiments for WT parasites, Δtgfnt2 parasites, Δtgfnt3 parasites, and Δtgfnt1 parasites complemented with tgfnt1 or tgfnt3.