Figure 4.

TgFNT1 transports l-lactate in Xenopus oocytes. l-[¹⁴C]lactate uptake into non-injected oocytes and oocytes expressing PfFNT or TgFNT1-HA was measured in the absence of compound (0.008% v/v DMSO; solvent control; white bars) and in the presence of the PfFNT inhibitor MMV007839 (4 µM; grey bars). The uptake of l-[¹⁴C]lactate is expressed as a percentage of that determined for TgFNT1-HA in the absence of MMV007839. The data are averaged from four independent experiments (using oocytes from different frogs), within which measurements were made from 7 to 10 oocytes per treatment. The symbols show the data from each experiment and the bars show the mean ± SD. Where not shown, error bars fall within the symbols. The data were tested for statistical difference from the non-injected control (black asterisks). The data obtained in the presence or absence of MMV007839 were also compared for each oocyte type (grey asterisks). ***P < 0.001 (one-way ANOVA with post hoc Tukey test); other comparisons did not show significant differences.