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. 2021 Mar 25;13:12. doi: 10.1038/s41368-021-00115-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Primary culture, immunocytochemistry identification, and RNA sequencing of NFs and CAFs in OSCC. a The phenotypes of NFs, CAFs, and Cal-27 cells were determined by staining for specific markers of stromal mesenchymal cells (vimentin, FSP-1) and epithelial cells (cytokeratin, CK) via immunocytochemistry. Scale bars: 50 μm. Representative images from each group are presented. b The phenotypes of NFs and CAFs were determined by staining for specific markers of CAFs (α-SMA, FAP, and FDGFR-β) via immunocytochemistry. Scale bars: 50 μm. c Heatmap of the differentially expressed lncRNAs between CAFs and NFs. n = 3, P < 0.05, fold change >2. d Bubble chart showing the enriched KEGG pathway analysis terms of differentially expressed genes between CAFs and NFs