Fig. 5.
FGF1ΔHBS suppresses Nur77 expression and activates AMPK in the hearts of db/db mice. a FPKM values of Nur77 gene in the RNA-Seq analysis. n = 3. b Nur77 mRNA levels confirmed by qPCR. n = 6. c Western blot analysis (left panel) and densitometric quantification (right panel) of Nur77 in the cardiac tissues. GAPDH was a loading control. n = 6. d Western blot analysis (left panel) and densitometric quantification (right panel) of Drp1, Nur77 and Cyt C in cardiac tissues of each group. HSP60 and GAPDH were loading controls. n = 6. e Western blot analysis (left panel) and densitometric quantification (right panel) of p-ACC, ACC, p-AMPK, AMPKα2 and SIRT1 in the cardiac tissues. GAPDH was a loading control. n = 6. Data were mean ± SEM; **P < 0.01, ***P < 0.001