Figure 4.
JAML promoted GC cell migration and proliferation by activating p38 signaling pathway. (A) The effect of JAML silencing on the phosphorylation of p38, ERK and JNK. (B–D) Quantitative analysis of (A) n=3, unpaired t test, *P < 0.05, compared with siNC group; ns, P > 0.05, compared with siNC group. (E) The effect of SB-203580 on p38 phosphorylation. (F) Quantitative analysis of (E) n=3, unpaired t test, **P < 0.01, compared with DMSO group. (G) Transwell migration assay to evaluate the effect of SB-203580 on cell migration in MKN-28 cells. (H) Quantitative analysis of (G) n=9, unpaired t test, ***P < 0.001, compared with DMSO group. (I) EdU incorporation assay to assess the effect of SB-203580 on cell proliferation in MKN-28 cells. (J) Quantitative analysis of (I) n=9, unpaired t test, ****P < 0.0001, compared with DMSO group.