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. 2021 Mar 11;11:595609. doi: 10.3389/fonc.2021.595609

Figure 1.

Figure 1

CircCHST15 expression was up-regulated in lung cancer and the gene was mainly expressed in the cytoplasm. (A) The expression of CircCHST15 in lung cancer tissues and adjacent normal tissues was detected by RT-qPCR, GAPDH was used as an internal control (*** P < 0.001, vs. Ant). (B) The expression of CircCHST15 in lung cancer cells and normal bronchial epithelial cells was detected by RT-qPCR, GAPDH was used as an internal control (^^^ P < 0.001, vs. 16HBE). (C) The expressions of CircCHST15 and CHST15 were detected by RT-qPCR in H1395 and A549 cells in which RNAs were treated with or without RNase R, GAPDH was used as an internal control (### P < 0.001, vs. RNase R−). (D) Relative CircCHST15 expression in the cell cytoplasm or nucleus of H1395 and A549 cells was determined by RT-qPCR, GAPDH was used as the cytoplasmic internal control, and U6 was used as the nuclear internal control. (Ant, adjacent normal tissues).