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. 2021 Mar 11;11:595609. doi: 10.3389/fonc.2021.595609

Figure 2.

Figure 2

ShCircCHST15 inhibited tumor growth, the expressions of IFN-γ, TNF-β, IL-10, PD-L1, and regulated the T cell subtype in lung cancer model in mice. (A) The solid tumors were observed. (B) The weight of a solid tumor was calculated. (C, D) The level of CD4+ T cells in the blood (C) and tumor supernatant (D) of mice was quantified by the flow cytometer. (E, F) The level of CD8+ T cells in the blood (E) and tumor supernatant (F) of mice was quantified by the flow cytometer. (G, H) The level of Tregs in the blood (G) and tumor supernatant (H) of the mice was calculated based on the formula: Tregs+ (%) = [(CD3+ + CD4+ + CD25+ + total cell number)/CD3+ + total cells number] × 100%. (I, J) The proportion of immune cell subsets in peripheral blood and tumor was measured by flow cytometry. (K–M) The levels of IFN-γ (K), TNF-β (L), and IL-10 (M) in the blood of the mice were detected by ELISA. (N) The expression of PD-L1 in the mouse tumor tissues was detected by immunohistochemical. (O) The expression of CircCHST15 in the mouse tumor tissues was detected by RT-qPCR, GAPDH was used as an internal control. (***P < 0.001, vs. NC; ^^ P < 0.01, ^^^ P < 0.001, vs. mock).