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. 2021 Mar 11;11:595609. doi: 10.3389/fonc.2021.595609

Figure 5.

Figure 5

The effects of CircCHST15 on the expressions of miR-155-5p, miR-194-5p, and PD-L1 were reversed by miR-155-5p mimic and miR-194-5p mimic. (A, B) The expression of CircCHST15 in H1395 cells (A) and A549 cells (B) after transfection was determined by RT-qPCR, GAPDH was used as an internal control. (C, D) The expressions of miR-155-5p and miR-194-5p in H1395 cells (C) and A549 cells (D) after transfection were determined by RT-qPCR, U6 was used as an internal control. (E, H) The gene expressions of PD-L1 in H1395 cells (E) and A549 cells (H) after transfection were determined by RT-qPCR, GAPDH was used as an internal control. (F, G, I, J) The protein expressions of PD-L1 in H1395 cells (F, G) and A549 cells (I, J) after transfection were determined by western blot, GAPDH was used as an internal control. (*** P < 0.001, vs. NC+MC; ^ P < 0.05, ^^^ P < 0.001, vs. circ+MC; ### P < 0.001, vs. NC+miR-155-5p; &&& P < 0.001, vs. NC+miR-194-5p).