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. 2021 Mar 11;11:595609. doi: 10.3389/fonc.2021.595609

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Copyright © 2021 Yang, Jia, Wang, Yang, Du, Luo, Li and Zhu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CircCHST15 regulated the expressions of PD-L1, Ki67, PCNA, miR-155-5p, and miR-194-5p in the mouse tumor tissues. (A) The expression of PD-L1 in the mice tumor tissues was detected by immunohistochemical assay. (B, C) The levels of PD-L1, Ki67, and PCNA in the tumor tissues were quantified by Western blot, GAPDH was used as an internal control. (D) The expression of CircCHST15 in the mouse tumor tissues was detected by RT-qPCR, GAPDH was used as an internal control. (E) The expressions of miR-155-5p and miR-194-5p in the mouse tumor tissues were detected by RT-qPCR, GAPDH was used as an internal control. (⁺⁺⁺ P < 0.001, vs. NC; ^*** P < 0.001, vs. Control; ^{^^^} P < 0.001, vs. mock; ^### P < 0.001, vs. CircCHST15; ^& P < 0.05, ^&&& P < 0.001, vs. Anti-PD-L1).