Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 11;11:642271. doi: 10.3389/fcimb.2021.642271

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Alonso, Carloni, Gonçalves, Martinez Peralta, Chesta, Pezza, Tavernelli, Motta and Serra

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

ATAT-HA colocalizes with acetylated α-tubulin in the cytoskeleton and flagella of epimastigotes. (A) Immunolocalization of ATAT-HA with rat monoclonal anti-HA antibodies and mouse monoclonal anti-acetylated α-tubulin (anti-AcTub) in isolated cytoskeletons and flagella of Dm28c pTcINDEX-GW ATAT-HA epimastigotes induced with 0.5 μg/ml tetracycline for 24 h. (B) Extracts enriched in cytoskeletal and flagellar proteins were analyzed by Western blot with rat monoclonal anti-HA antibodies, rabbit polyclonal anti-TcATAT antibodies, mouse monoclonal anti-acetylated α-tubulin (anti-AcTub) and anti α-tubulin (anti- αTub). SN1, soluble protein extracts; SN2, soluble cytoskeletal and flagellar protein extracts; P, insoluble cytoskeletal and flagellar protein extracts.