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Lewis A bearing complex N-glycans are present on recombinantly produced plant proteins. KORRIGAN (A), g6145 (B), and CEBIP (C) were transiently expressed in leaves of wild-type and ΔXT/FT plants of N. benthamiana and purified via their fused tag. Expression of proteins was confirmed via immunoblotting using an antibody directed against the fused tag (GFP for KORRIGAN and mRFP for g6145 and CEBIP). Presence of Lewis A was shown using the JIM84 antibody.
