FIGURE 4.

Comparison of the time course of wound closure with the anti-MT drugs in a 12-h interval shows that the untreated cells HaCaT, 3T3, and A549 advance significantly into a wound, while the scratch area is closing slowly in the presence of nocodazole, Taxol, and vinorelbine (A). (B) Enlargement of a scene for the wound edge of the control cells and cells treated by nocodazole (300 nM). HPF and 3T3 cells in normal conditions form long lamellae protrusions, while lamellas are significantly shorter after treatment with nocodazole. The A549 shows a similar behavior. After nocodazole treatment, no lamellae appear at the wound edge. Cells arrested in mitosis are evident under nocodazole treatment in all three cultures. Bar = 100 μm (for enlargement).