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. 2021 Mar 11;9:636327. doi: 10.3389/fcell.2021.636327

FIGURE 5.

FIGURE 5

Dexmedetomidine regulates ERK1/2-mediated mitochondrial fission. (A) Effects of dexmedetomidine on the phosphorylation of Drp1 after sepsis in VECs detected by Western Blotting, n = 3. (B) Effects of dexmedetomidine on the mitochondrial translocation of Drp1 after sepsis in VECs, n = 3. (C,D) The co-location of Drp1 and mitochondria of VECs in each group (Bar, 25 μm), n = 3. (E) The length of mitochondria of VECs in each group. (F) Drp1 protein phosphorylation network (PPN) predicted by iGPS 1.0 software. (G) Effects of dexmedetomidine on the phosphorylation of ERK1/2 after sepsis in VECs, n = 3. (H) Effects of dexmedetomidine and ERK1/2 inhibitor on the phosphorylation of ERK1/2 after sepsis in VECs, n = 3. (I) Mitochondrial morphology in PD98059-treated VECs after sepsis (Bar, 15 μm), n = 3. (J,K) Effects of PD98059 on the TER and FITC-BSA infiltration rate of VEC monolayers after sepsis, n = 3. Normal, normal group; LPS, lps group; Dex, dexmedetomidine group. PD98059: ERK1/2 inhibitor group. ∗∗P< 0.01, as compared with normal group; ##P < 0.01, as compared with lps group.