Table 2.
Effect of Green tea extract supplementation (1.0 ng ml−1) to cryoprotective media on sperm motility and DNA integrity in postthaw semen
| Parameter | Fresh | CP | CP+GTE |
|---|---|---|---|
| Total motility (%) | 45.5 (41.5–49.4) | 7.2 (6.3–10.8)a | 9.7 (8.9–14.8)a,b |
| Progressive motility (%) | 40.3 (35.6–43.5) | 5.5 (5.1–9.1)a | 7.9 (6.9–11.2)a,b |
| Kinetic motility | |||
| ALH (µm s−1) | 4.2 (3.7–4.3) | 4.3 (3.9–5.0) | 3.9 (3.5–4.4) |
| LIN (%) | 53.6 (52.5–56.1) | 47.9 (40.3–49.0)a | 42.6 (40.0–48.1)a |
| STR (%) | 77.5 (75.5–79.6) | 71.6 (64.7–73.6)a | 68.6 (66.1–74.0)a |
| VAP (µm s−1) | 40.3 (36.4–43.8) | 29.5 (26.8–37.1)a | 28.4 (25.9–32.6)a |
| VCL (µm s−1) | 59.3 (53.6–64.2) | 49.1 (45.0–66.3)a | 50.6 (44.2–54.5)a |
| VSL (µm s−1) | 33.5 (29.5–36.6) | 21.2 (19.2–25.6)a | 19.3 (18.5–24.9)a |
| DNA fragmentation (%) | 19.0 (17.9–24.3) | 28.0 (26.6–33.8)a | 24.0 (23.4–30.2)a,b |
| ROS (mean fluorescence) | NA | 7.2 (6.0–7.8) | 7.1 (6.2–8.5) |
Comparisons between groups performed using Friedman (RM) ANOVA test, except for ROS (Wilcoxon signed-rank test was used). Statistical significance indicated as: aP<0.05, CP or (CP + GTE) versus Fresh; bP<0.05, (CP + GTE) versus CP. Value given as median (95% CI). Fresh: fresh ejaculate; CP: cryopreservative only (control); CP + GTE: cryopreservative supplemented with 1.0 ng ml−1 green tea extract; ALH: mean amplitude of lateral head displacement; LIN: linearity; STR: straightness; VAP: average path velocity; VCL: curvilinear velocity; VSL: straight-line velocity; ROS: reactive oxygen species; NA: not analyzed; RM: repeated measures; CI: confidence interval