Table 3.
Effect of green tea extract supplementation (1.0 ng ml−1) to cryoprotective media on sperm motility and DNA integrity in patients with different semen quality
| Parameter | Fresh | CP | CP + GTE |
|---|---|---|---|
| Normal motility (n=33) | |||
| Total motility (%) | 51.9 (47.8–53.9) | 7.6 (7.4–12.9)a | 10.6 (9.8–16.3)a,b |
| Progressive motility (%) | 46.1 (42.6–48.4) | 6.5 (6.0–10.9)a | 9.7 (7.7–12.4) a,b |
| Abnormal motility (n=12) | |||
| Total motility (%) | 27.1 (22.5–35.9) | 2.8 (1.6–6.2)a | 3 (1.6–15.0)a |
| Progressive motility (%) | 22.6 (17.2–26.1) | 1.4 (0.8–5.2)a | 1.8 (1.1–11.3)a |
| Low DNA damage (n=33) | |||
| DNA fragmentation (%) | 18 (13.6–19.2) | 25 (20.4–27.8)a | 22 (18.3–24.9)a,b |
| High DNA damage (n=12) | |||
| DNA fragmentation (%) | 30 (27.4–50.8) | 41.2 (37.0–60.6)a | 34.7 (32.6–56.3)a,b |
Normal and abnormal sperm motility (WHO 5th edition). Low DNA damage: damage <25%; high DNA damage: damage ≥25%. Statistical significance levels were measured by Friedman (RM) ANOVA test followed by Wilcoxon signed-rank test, and indicated as: aP<0.05, CP or (CP + GTE) versus Fresh; bP<0.05, (CP + GTE) versus CP. Value given as median (95% CI). Fresh: fresh ejaculate; CP: cryopreservative only (control); CP + GTE: cryopreservative supplemented with 1.0 ng ml−1 green tea extract; RM: repeated measures; CI: confidence interval