Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 2;23(2):197–204. doi: 10.4103/aja.aja_56_20

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: ©The Author(s)(2020)

This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.

PMC Copyright notice

The impact of the MNS1 variant. (a) Evolutionary conservation analysis of the MNS1 variant among different species. (b) The positions of the identified variant (red) and previously reported mutations (black) in MNS1 in patients are shown. The trichohyalin–plectin–homology (TPH) domain (http://smart.embl-heidelberg.de/) in MNS1 is indicated in blue. (c) Western blot analysis shows that the MNS1 protein (approximately 60 kDa) is absent from the proband's sperm. (d) Immunostaining analysis shows that MNS1 is localized in the flagella of the sperm in the NC, but was absent in the sperm of the proband (IV-1; n = 120). In the NC, the staining of MNS1 in the midpiece is prominent, whereas that in the principal and end piece is distributed discretely with distinct dots. Scale bars = 5 μm. MNS1: meiosis-specific nuclear structural 1; α-tub: alpha-tubulin; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; NC: normal control.