Supplementary Table 2.
Primers used in Sanger sequencing of meiosis-specific nuclear structural 1 heterozygous variants
| Patient | Variant | Primer | Primer sequences | Exon | Product size |
|---|---|---|---|---|---|
| P1/P2 | c.G149A | Forward | ATCGTAGGGTTCCAAAAGGAG | 2 | 384 |
| Reverse | CCCAGGTCTTTACGATTACACC | ||||
| P3/P4 | c.A454G | Forward | CCGCTGTATTCCCTCTTTGAGTA | 4 | 429 |
| Reverse | TGGTCATTCTAAGCAGCAATGT | ||||
| P5 | c. 846_848del | Forward | CTGTACTTGTTATTGCTTTTCAGGG | 6 | 414 |
| Reverse | TCCTGGTATAATTCTTGTCGCAC | ||||
| P6 | c.G932A | Forward | AATGAATGCAATGCGAAGGT | 7 | 441 |
| Reverse | CTTCTCTACATTAATCATTCTTTCC |