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. 2021 Mar 11;12:627819. doi: 10.3389/fendo.2021.627819

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Copyright © 2021 Dijkstra, van den Berg, Weeber, van de Haar, Velds, Kaing, Peters, Eskens, de Groot, Tesselaar and Voest

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mismatch repair status of GEP-NEC organoid lines. Staining of slides from NEC-01010 (P7 and P11), NEC-01013 (P13), and NEC-02002 organoids (P15), as well as the original tumor of NEC-02002, for mismatch repair proteins MLH1, PMS2, MSH2, and MSH6. Note 100% nuclear expression of MHS2 and MSH6 but absence of nuclear staining of MLH1 and PMS2 in both organoids and original tumor of NEC-02002. The brown halo on the cell border is a specific staining caused by residual Geltrex in organoid slides. Scale bar = 100 µm.