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. 2021 Mar 11;11:636373. doi: 10.3389/fonc.2021.636373

Figure 3.

Figure 3

ODC-MPI-2 simultaneously perturbs the polyamine pathway and the cAMP pathway. (A) ODC-MPI-2 inhibited the synthesis of polyamines. Cells were treated with the indicated doses of different inhibitors for 72 h. The cells were lysed, and the polyamine amounts were quantified with an HPLC assay. The polyamine levels were expressed as the ratio between the polyamines and the amount of total proteins. The data are shown as mean ± SD (n = 3). *, ** and *** indicate p < 0.05, p < 0.01, and p < 0.001 respectively compared to the control group. #, ## and ### indicate p < 0.05, p < 0.01, and p < 0.001 respectively compared to the DFMO group. (B) ODC-MPI-2 stimulated the production of cAMP. Cells were serum-starved and treated for 6 h with the indicated doses of different inhibitors. The cells were lysed, and the cAMP level was quantified using a Direct Cyclic AMP Enzyme Immunoassay Kit. Data are shown as mean ± SD (n = 3). ** and *** indicate p < 0.01 and p < 0.001 respectively compared to the control group. # and ## indicate p < 0.05 and p < 0.01 respectively compared to the DFMO group. ns, not significant.