Figure 2.

Desialylation of FHR-1 and FHR-3 in patient H837 by 2D-WB analysis. FHR1 and FHR3 partially purified from sp1 and sp2 from patient H837 and from a control individual were subjected to 2D-electrophoresis and Western-blot with polyclonal antibodies. The characteristic ladder of FHR-3 spots (each having different Mw and isoelectric point) appeared as a few basic spots of lower Mw in the patient sample obtained 1 day after SP-HUS diagnosis (H837 sp1), and was recovered in the sample drawn 3 months later (H837 sp2). A similar situation applies to FHR-1. The small boxes within the gel images indicate the neuraminidase activity detected in samples sp1 and sp2 from patient H837.