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Binding of FH and dFH to surface-bound C3b. Binding of increasing concentrations of FH and dFH to microtitre plates coated with 400 ng of C3b. Upon addition of a polyclonal antibody which recognizes FH and dFH, absorbance was read at 420 nm, and the maximum binding observed was set as 100%. The two curves represent the mean ± SD from four independent ELISA experiments. **P < 0.01; ***P < 0.001.
