Figure 8.

Haemolytic assays on sheep erythrocytes. (A) Lysis of sheep erythrocytes by the serum from an aHUS patient carrying the mutation W1183L in FH (left panel). The lysis generated by 12.5 μL of patient's serum (about 50%) could be prevented by addition of exogenous FH or dFH, but dFH was less effective (right panel). (B,C) Lysis of sheep erythrocytes was induced by adding increasing amounts of monoclonal antibodies OX24 (B) or C18 (C) to 20 μL of NHS (left panels). MoAb OX24 recognizes FH SCR4 domain, and MoAb C18 recognizes FH SCR20 domain; both antibodies recognize similarly FH and dFH by Western-blot (insets). The lysis induced by 1.6 μg of OX24 (about 70%) or C18 (about 60%) could be inhibited by adding increasing concentrations of FH or dFH, although dFH was less effective (right panels). All curves represent the mean ± SD from three independent experiments. *P < 0.05; **P < 0.01.