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Permutation tests of the PLS-DA model for CG vs Control, CG vs GC, and GC vs Control in the (A) ESI+ and (B) ESI− ion modes. Random permutations (a total of 200) were used to evaluate whether a possibility of overfitting in the PLS-DA model. The statistical parameters R2X0, Q2X0, R2Y, and Q2Y were used for the analysis of the multivariate models.
