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. 2021 Mar 11;9:648382. doi: 10.3389/fbioe.2021.648382

TABLE 1.

Sugar alcohols and functional sugars produced by engineering Y. lipolytica.

Products Carbon source Genetic engineering strategy Cultivation Strategy Titer References
Glycerol The overexpressed codon optimized bacterial hemoglobin from Vitreoscilla stercoraria was used Bioreactor culture 55.75 g/L Mironczuk et al., 2019
Glycerol Overexpressed TKL1 and GUT1, Disruption of EYK1 Bioreactor culture 80.6 g/L Carly et al., 2017; Carlya et al., 2017
Glycerol Ultraviolet (UV) mutagenesis and optimal C:N ratio Chemostat culture 113.1 g/L Rakicka et al., 2017a
Erythritol Glycerol The overexpression of Erythrose reductase encoding gene YALI0F18590gwas used Batch culture 44.44 g/L Janek et al., 2017
Glycerol The four genes ZWF1, TAL1, GND1, and TKL1 were functionally overexpressed Shake flask experiment 51.09 g/L Mironczuk et al., 2017
Glycerol The disruption of Gene encoding erythrulose kinase YALI0F01606g was used Batch bioreactor 35.7 g/L Carly et al., 2017; Carlya et al., 2017
Glycerol Span 20 surfactant was added Fed-batch culture 142 g/L Rakicka et al., 2017b
D-threitol Glucose The overexpression of xylitol dehydrogenase gene (Ss-XDH) from Scheffersomyces stipitis Culture broth 112 g/L Chi et al., 2019
Isomaltulose Sucrose Displaying sucrose isomerase encoding gene from Pantoea dispersa UQ68J (PdSIase) on the Y. lipolytica cell surface Fermentor 465 g/L Li et al., 2017
Sucrose gene encoding sucrose isomerase (SIase) from Pantoea dispersaUQ68J Fermentor 572.1 g/L Zhang et al., 2018
Trehalose Maltose Displaying encoding trehalose synthase gene (TreS) on the Y. lipolytica cell surface Bioreactor 219 g/L Li et al., 2016
Fructo-oligosaccharides Sucrose Erythritol producing Y. lipolytica cells containing displayed fructosyltransferase Bioreactor 480 g/L Zhang et al., 2016
Inulin The overexpression of optimized endo-inulinase gene from Aspergillus nigerwas used Bioreactor 546.6 g/L Han et al., 2017
Galacto-oligosaccharides lactose Displaying β-galactosidase on Y. liplytica cell surface Bioreactor 160 g/L An et al., 2016