Fig. 5.

Effects of a repeated dose exposure (14d and 28d) to different types of POP mixtures on proportion of diverse cell populations. (A) Quantification of nestin+, nestin+/Ki67+, MAP2+ and GFAP + cell percentages upon 14 or 28 day treatment with POP mixtures at the concentrations 0.5, 1, and 1000 × . (B) Representative immunocytochemical images of nestin (red) and Ki67 (green) (upper panels), and MAP2 (red) staining (lower panels) in solvent control culture and upon 28 day treatment with PerF only, Br only and Cl only mixtures at 1x concentration. Data are normalized to solvent control (0.1 % DMSO) and presented as mean ± S.E.M. with asterisks indicating statistical significance compared to solvent control (* p < 0.05, ** p < 0.01, *** p < 0.001). (For interpretation of the references to colour in the Figure, the reader is referred to the web version of this article).