KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| Library Efficiency™ DH5α™ Competent Cells | ThermoFisher Scientific | Cat# 18263012 |
| E. coli NiCo21(DE3) | BioRad | Cat# 1523920 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Atto520, free acid | Sigma-Aldrich | Cat# 70706-1MG-F |
| MetafectenePro | Biontex | Cat# T040-5.0 |
| Fluo-8 AM | Abcam | Cat# ab142773 |
| Pluronic F127 | Sigma-Aldrich | Cat# P2443-250G |
| Probenecid | Sigma-Aldrich | Cat# P8761-25G |
| myo-[2-3H]-inositol | Perkin Elmer | Cat# NET1156001MC |
| 1,2-diheptanoyl-sn-glycero-3-phosphocholine (DHPC) | Avanti Polar Lipids | Cat# 850306 |
| 1,2-dimyristoyl-sn-glycero-3-phophocholine (DMPC) | Avanti Polar Lipids | Cat# 850345 |
| 1,2-dimyristoyl(d54)-sn-glycero-3-phosphocholine (DMCP-d54) | Avanti Polar Lipids | Cat# 860345 |
| 1,2-dimyristoyl-sn-glycero-3-phophatidylserine (DMPS) | Avanti Polar Lipids | Cat# 840033 |
| BioBeads SM-2 | BioRad | Cat# 1523920 |
| Ghrelin and 13C variants | (Vortmeier et al., 2015) | NA |
| [Dpr3-Oct; Dpr16-atto520]-Ghrelin | (Schrottke et al., 2017) | NA |
| Deposited Data | ||
| Ensemble of Ghrelin-GHSR | PDB-Dev | PDBDEV_00000024 |
| Ghrelin Peptide 13C Chemical Shift Data | BMRB | 27600 |
| Experimental Models: Cell Lines | ||
| HEK293 | DSMZ | ACC305 |
| COS-7 | ATCC | CRL-1651 |
| Oligonucleotides | ||
| Primers for mutagenesis, see Table S5 | ||
| Recombinant DNA | ||
| Plasmid: pV2_GHSR1a_eYFP | (Els et al., 2012) | NA |
| Plasmid: pET41b(+)-GHSR_M1-T366_LE_8xHis (ATGTGGAACGCGACGCCCAGCGAAGAGCCGGGGTTCAACCTCACACTGGCCGACCTGGACTGGGATGCTTCCCCCGGCAACGACTCGCTGGGCGACGAGCTGCTGCAGCTCTTCCCCGCGCCGCTGCTGGCGGGCGTCACAGCCACCTGCGTGGCACTCTTCGTGGTGGGTATCGCTGGCAACCTGCTCACCATGCTGGTGGTGTCGCGCTTCCGCGAGCTGCGCACCACCACCAACCTCTACCTGTCCAGCATGGCCTTCTCCGATCTGCTCATCTTCCTCTGCATGCCCCTGGACCTCGTTCGCCTCTGGCAGTACCGGCCCTGGAACTTCGGCGACCTCCTCTGCAAACTCTTCCAATTCGTCAGTGAGAGCTGCACCTACGCCACGGTGCTCACCATCACAGCGCTGAGCGTCGAGCGCTACTTCGCCATCTGCTTCCCACTCCGGGCCAAGGTGGTGGTCACCAAGGGGCGGGTGAAGCTGGTCATCTTCGTCATCTGGGCCGTGGCCTTCTGCAGCGCCGGGCCCATCTTCGTGCTAGTCGGGGTGGAGCACGAGAACGGCACCGACCCTTGGGACACCAACGAGTGCCGCCCCACCGAGTTTGCGGTGCGCTCTGGACTGCTCACGGTCATGGTGTGGGTGTCCAGCATCTTCTTCTTCCTTCCTGTCTTCTGTCTCACGGTCCTCTACAGTCTCATCGGCAGGAAGCTGTGGCGGAGGAGGCGCGGCGATGCTGTCGTGGGTGCCTCGCTCAGGGACCAGAACCACAAGCAAACCGTGAAAATGCTGGCTGTAGTGGTGTTTGCCTTCATCCTCTGCTGGCTCCCCTTCCACGTAGGGCGATATTTATTTTCCAAATCCTTTGAGCCTGGCTCCTTGGAGATTGCTCAGATCAGCCAGTACTGCAACCTCGTGTCCTTTGTCCTCTTCTACCTCAGTGCTGCCATCAACCCCATTCTGTACAACATCATGTCCAAGAAGTACCGGGTGGCAGTGTTCAGACTTCTGGGATTCGAACCCTTCTCCCAGAGAAAGCTCTCCACTCTGAAAGATGAAAGTTCTCGGGCCTGGACAGAATCTAGTATTAATACACTCGAGCACCACCACCACCACCACCACCAC) | Modified from Schrottke et al. (2017) | N/A |
| Software and Algorithms | ||
| TopSpin | Bruker | https://www.bruker.com/products/mr/nmr/nmr-software/software/topspin/overview.html |
| Rosetta | (Leaver-Fay et al., 2011) | https://www.rosettacommons.org/ |
| GraphPad Prism | GraphPad Software, Inc. | www.graphpad.com/scientific-software/prism/ |
| ProShift | (Meiler, 2003) | http://www.meilerlab.org/index.php/servers/show?s_id=9 |
| PyMol | Schrödinger | http://www.pymol.org/ |