Figure 2.

(a) Spatial resolution allows recording monochromatic images, such as the distribution I(x,y) of one type of fluorophore within one cell. (b) Temporal resolution allows recording intensity fluctuations I(t) at one point, which with correlation analysis enables diffusion measurements of fluorophores. (c) Spectral resolution permits discrimination of the fluorescence I(λ) of fluorophores emitting at different wavelengths, λ. (d) Spatial and temporal resolution taken together allow recording of monochromatic movies, such as the movement of one type of fluorophores within one cell. (e) Spatial and spectral resolution taken together allow recording multicolor images, such as the distribution of different fluorophores in one cell. (f) Temporal and spectral resolution together enable multiplexed recording of intensity variations of multiple fluorophores. (g) Taking spatial, temporal, and spectral resolution together makes it possible to record multicolor movies, such as recording the movement of multiple different fluorophores in one cell.