Figure 5.

Unrestrained action of BBT on various drug-resistant factors. (A) Molecular docking of BBT (green) and LBT (blue) in P-gp receptor protein (brown). Interaction of (B) BBT and (C) LBT with amino acids of P-gp. (D) Western blots showing the expression of cleaved caspase 3, p-HSP27, and NFκB in HeLa cells after treatment with 12.5 μM BBT after various time points of treatment. The α-tubulin expression has been used as a loading control. (E) Cancer cell recurrence efficiency using MTT assay: MTT assay after treatment of BBT for 48 h in one set or 24 h with and without BBT in SiHa cells in another set. All experiments have been performed thrice (n = 3). P-gp: P-glycoprotein, HSP27: heat shock protein 27, and NFκB: nuclear factor kappa B.