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. Author manuscript; available in PMC: 2021 Mar 25.
Published in final edited form as: Anticancer Drugs. 2009 Jul;20(6):425–436. doi: 10.1097/CAD.0b013e32832ae55f

Fig. 2.

Fig. 2

Naamidine A (NA)-induced G1 cell cycle arrest. (a) Fluorescence-activated cell sorter (FACS) cell cycle analysis after NA treatment of A431 cells for 12 h. When treated with 30 μmol/l NA, an increase in percentage of cells in the G1 phase of the cell cycle occurs with a concomitant withdrawal of cells in the S phase. The t-test statistical analysis was used to compare the changes in percentage G1 and percentage S phase populations to untreated control cells, which yielded a P ≤ 0.005 (*). (b) Western blot analysis of cell cycle inhibitor p21waf1/cip1 protein expression levels after NA (30 μmol/l) or epidermal growth factor (EGF) (2 nmol/l) treatment. Induction of p21 protein levels occurs within 1 h of NA treatment with maximum induction after 2 h. Full-length western blots are presented in Supplemental Figure S1.