Fig. 2.

a PCA plots before (left) and after (right) RUV treatment, showing the separation between wild type and mutant larvae across principle components PC1 and PC2. Each sample is labelled by pair (i.e. B, D, F, G, H, or I). b A volcano plot highlighting identified DE genes in red. The DE genes with absolute log₂FC > ±1.2 are labelled on the plot. The black vertical lines indicate absolute log₂FC = ±0.5. c A plot of percent variation summarizing the contribution of each variable