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. 2021 Mar 23;218(5):e20201974. doi: 10.1084/jem.20201974

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© 2021 Chen et al.

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Figure S5. — Knockdown of DEK in human primitive hematopoietic cells decreases quiescence. (A) Relative mRNA expression of NCoR1 and HDAC3 in freshly sorted HSCs of Dek^fl/fl and Dek-cKO mice (n = 3). (B) Gel electrophoretic analysis of DNA recovered from MNase-digested nucleus of Lin⁻c-Kit⁺ cells, which were sorted and cultured in vitro, with treatment of RGFP-966 (5 µM) or GNE-049 (500 nM) for 24 h. (C) Analysis of quiescence of hHSPCs by Ki-67 + DAPI staining (n = 4–5). Human BM CD34⁺ cells (hHSPCs) were cultured in vitro and transduced to express control shRNA (shControl) or shRNA targeting DEK (either of two constructs: shDEK-1 and shDEK-2). (D) Relative mRNA expression for DEK, AKT1, and AKT2 in hHSPCs (n = 4). Error bars represent means ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; Student’s t test or one-way ANOVA. Data are representative of three independent experiments.