Fig. 4.

SARS-CoV-2 Nsp6, Orf6 and Orf7a transgene expression in Drosophila results in locomotion defect and reduced mitochondria. a Quantification of climbing ability in SARS-CoV-2 Nsp6, Orf6 and Orf7a transgenic flies. N = 30 flies per group. b Representative images of typical (wild type, w¹¹¹⁸) and “held-up” wing phenotype in SARS-CoV-2 Nsp6, Orf6 and Orf7a expression flies. c Quantification of flies with “held-up” wing phenotype (i.e. % Wing Defect). Four replicates, each replicate N = 50 flies per group. d Representative images of indirect flight muscle (labeled with Phalloidin, red) and mitochondria (labeled with ATP5A, green) morphology in SARS-CoV-2 Nsp6, Orf6 and Orf7a flies. e Quantification of the number of mitochondria in the fly indirect flight muscle of both wings, normalized based on wild type (w¹¹¹⁸) counts. N = 10 flies per group. The results are presented as mean ± SD. Statistical significance (*) is defined as P < 0.05