Fig. 2.

Methylation of IRF8 in BC cell lines and samples. a Methylation of the IRF8 promoter was evaluated by methylation-specific PCR (MSP) in the BC cell lines MDA231 and MCF7 (M: methylated; U: unmethylated). b Expression of IRF8 evaluated by western blot in MDA231 and MCF7 cells. c Correlation of IRF8 promoter methylation and gene expression in BC samples. Data are from The MethHC database tool (http://methhc.mbc.nctu.edu.tw/). Gene expression value was obtained from RNA Seq RPKM (reads per kilobase per million mapped reads) values in TCGA Data Portal. Data shown in a and b are representative of two experiments performed