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Multiparameter cytometric image analysis for quantification of multiplex IHC. Image cytometry-based cell population analyses for the lymphoid and myeloid biomarker panels are shown in a and b, respectively. Gating thresholds for qualitative identification were determined based on data in negative controls. TMA of BC samples were stained with the lymphoid (c) and myeloid (d) biomarker panels by pseudo-coloring. Scale bars, 50 μm
