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. 2021 Mar 24;12:208. doi: 10.1186/s13287-021-02264-2

Fig. 1.

Fig. 1

Characterization of hiPSC-CMs. a Immunostaining of hiPSC-CMs for cTnT, CX43, and α-actinin. b Q-PCR of cardiac marker genes MYH6, MYL7, and TNNT2 during differentiation. The abscissa represents the number of days of differentiation. n = 3; the means ± SEM are shown. ****P < 0.0001. c TEM images of hiPSC and hiPSC-CMs, myofibrils (yellow arrow), Z-disks (red arrow), and mitochondria (blue arrow). d Flow cytometry analysis of the hiPSC-CMs by cTnT measurements demonstrated cardiac cell purity greater than 98%