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. 2021 Mar 24;12:208. doi: 10.1186/s13287-021-02264-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — NRF2 promotes the morphology and structural maturation of hiPSC-CMs. a Q-PCR to detect the expression of KEAP1 in siKEAP1 and siNC hiPSC-CMs. b Western blot (left) and quantification (right) to detect the expression of KEAP1, NRF2, and phosphorylated NRF2 (PS40-NRF2) in siKEAP1 and siNC hiPSC-CMs. n = 3. c mRNA expression of ion channels in siKEAP1 and siNC hiPSC-CMs. Gene expression is normalized first to GAPDH and then normalized to siNC. d α-Actinin (green) and Hoechst (blue) staining of representative siKEAP1 and siNC hiPSC-CMs (scale bar = 10 μm). e–i Compared with siNC cells, siKEAP1 cells showed a significant increase in e perimeter, f area, g sarcomere length, and i multinucleation ratio (n = 3) and h a decrease in the circularity index (n > 10 cells per condition, three biological replicates). The means ± SEM are shown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001