Figure 2.
Effect of endogenous TF on MDA-MB-231 adhesion to HUVECs. (a, b)Three breast cancer cells (MDA-MB-231 cells, HS-578 T cells and MCF-7 cells) adhere to HUVECs wereexamined by static adhesion assay, and the adhesion of three breast cancer cells to endothelial cells blocked by TF antibodywere examined by static adhesion assay, bar = 20 μm, the bar graph represents the adhesion rate ((n = 4, ***P < 0.001 vs. MDA-MB-231 cells, no significant (ns). (c, d) Three breast cancer cells (5 × 105 cells/mL) adhere to endothelium under shear (2dynes/cm2)were examined by Parallel plate flow assay, and the adhesion of three breast cancer cells to endothelial cells blocked by TF antibody were examined usingParallel plate flow assay, bar = 20 μm, the number of adhesion cells was quantified by Image J (right, n ≥ 5, ***P < 0.001 vs. MDA-MB-231 cells, (ns (no significantly)