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. 2021 Mar 18;15(1):74–83. doi: 10.1080/19336918.2021.1898709

Figure 4.

Figure 4.

Effect of TF and α3β1 integrin on the MDA-MB-231cells adhesion to HUVECs.

(a)Expression of adhesion molecules (β1integrin, α3 integrin) on HUVECs were analyzed by flow cytometry. (b) Expression of TF and fibronectin on MDA-MB-231cells were analyzed by flow cytometry. (c)The effect of TF and α3β1integrin on the cell-cell adhesion between MDA-MB-231cells and HUVECswas examined by static adhesion, MDA-MB-231 cells(4 × 105cells/mL) pretreated with TF antibody (20 μg/mL) and fibronectin antibody(10 μg/mL) respectively, HUVECs pretreated with β1integrinantibody (1:100 dilution) and α3 integrin antibody(10 μg/mL) respectively,bar = 10 μm. The bar graph right represents the adhesion rate (n = 5, ***P < 0.001 vs. untreated cells, ### P < 0.001 vs.TF Ab).(d) The effect of TF and α3β1integrin on the cell-to-cell adhesion between MDA-MB-231cellsand HUVECswas examined by Parallel plate flow assay, MDA-MB-231 cells(1 × 106cells/mL) pretreated with TF antibody (20 μg/mL) and fibronectin antibody(10 μg/mL) respectively, HUVECs pretreated with β1integrinantibody (1:100 dilution), α3 integrin antibody(10 μg/mL) and E-selectin (10 μg/mL) respectively,bar = 10 μm. The bar graph right represents the number of adhesion cells was quantified by Image J(n ≥ 5, **P < 0.05, ***P < 0.001 vs. untreated cells, ### P < 0.001 vs.TF Ab).