Figure 2.

Cell binding and internalization of Dab-Fc 2 × 3 antibody using different tumor cell lines. Binding of the bispecific Dab-Fc 2 × 3 molecule to breast cancer cell lines (MCF-7 (a), BT474 (b), SKBR3 (c)) and the gastric cancer cell-line NCI-N87 (d). Bound antibodies were measured using PE-labeled anti-human Fc secondary antibody. The parental antibodies (trastuzumab and IgG 3–43) were included as a control. Mean ± SD, n = 3. e) and f) internalization of the pHrodo-labeled antibodies was tested using the gastric cancer cell-line NCI-N87 at 37°C and 4°C. Mean ± SD, n = 1, statistics: t-test. *p < .05