Figure 3.

Western blot analysis of receptor phosphorylation and downstream signaling: NCI-N87 cells were starved overnight before treatment with antibodies (trastuzumab, IgG 3 43, combination of trastuzumab and IgG 3–43, or Dab-Fc 2 × 3) for 1 h. After incubation with the antibodies, cells were stimulated with 50 ng/ml HRG for 15 min. a) Subsequently, cell lysates were analyzed by western blot. Signals of unstimulated control cells (left lane) are from the same blot. b) Data shown as mean intensity of signals normalized to α-Tubulin. Mean ± SD, n = 2