Figure 5.

Inhibition of migration of NCI-N87 cells by Dab-Fc 2 × 3. Cells were serum starved (0.2% FCS) for 24 h and a scratch was introduced into confluent cell layer. Cells were then treated with rituximab (negative control), trastuzumab, IgG 3–43, a combination of trastuzumab and IgG 3–43 (each 50 nM), or Dab-Fc (100 nM). Cells were either left unstimulated (a) or were stimulated (b) with 25 ng/ml HRG. Relative wound density was measured for 24 h at 37°C. c) relative wound density after 5 h or 24 h of incubation. Mean ± SD, n = 3, statistics: one-way ANOVA. *p < .05, **p < .01, ***p < .001, ****p < .0001