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. 2021 Mar 11;11:632623. doi: 10.3389/fonc.2021.632623

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Copyright © 2021 Robinson, Davies, Darley and Tonks

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in glycolytic intermediates in AML derived cell lines with NOX2 KD or treated with DPI. Significant changes in glycolytic metabolites in (A) shTHP-1 and shNOMO-1 cells with NOX2 KD. (B) shTHP-1 and shNOMO-1 cells treated with DPI (100nM) for 24h. Data shows relative values following normalization to protein concentration, log transformation and imputation of missing values with the minimum observed value for each compound (n = 4). Box plots represent median quartile ranges, x represents mean value. * denotes p < 0.05 = as analyzed by Welch’s two sample t-test. THP-1 cells are color coded (blue) and NOMO-1 cells color coded green.