Table 1.
Exposure of AML cell lines to ROS is associated with global changes in sphingolipid metabolism.
| Biochemical name | THP-1 | NOMO-1 | Mv4;11 | |||
|---|---|---|---|---|---|---|
| NOX2 KD vs shControl | DPI vs shControl | NOX2 KD vs Control | DPI vs shControl | GOX 10 mU/ml vs Control | GOX 20 mU/ml vs Control | |
| N-palmitoyl-sphinganine (d18:0/16:0) | 0.68 | 0.49 | 1.30 | 0.81 | 2.27 | 2.88 |
| sphinganine | 0.82 | 0.47 | 1.10 | 0.30 | 1.06 | 0.99 |
| phytosphingosine | 0.65 | 0.80 | 0.81 | 0.63 | 1.12 | 1.13 |
| palmitoyl sphingomyelin (d18:1/16:0) | 0.80 | 1.22 | 0.85 | 0.98 | 1.09 | 0.89 |
| stearoyl sphingomyelin (d18:1/18:0) | 0.82 | 1.10 | 0.65 | 0.96 | 1.31 | 1.04 |
| sphingomyelin (d18:1/18:1, d18:2/18:0) | 0.89 | 1.16 | 0.61 | 0.96 | 1.39 | 1.14 |
| sphingosine | 0.62 | 0.37 | 0.70 | 0.30 | 1.13 | 1.00 |
| N-palmitoyl-sphingosine (d18:1/16:0) | 0.76 | 1.68 | 0.88 | 1.27 | 1.08 | 0.88 |
| sphingomyelin (d18:1/14:0, d16:1/16:0)* | 0.80 | 1.32 | 0.88 | 1.22 | 1.15 | 0.96 |
| sphingomyelin (d18:2/14:0, d18:1/14:1)* | 0.77 | 1.24 | 0.85 | 1.16 | 1.45 | 1.37 |
| sphingomyelin (d18:1/24:1, d18:2/24:0)* | 0.81 | 1.17 | 0.85 | 0.85 | 1.32 | 1.06 |
| sphingomyelin (d18:2/16:0, d18:1/16:1)* | 0.78 | 1.07 | 0.88 | 0.91 | 1.35 | 1.19 |
| sphingomyelin (d18:1/20:1, d18:2/20:0)* | 0.81 | 0.92 | 0.76 | 1.21 | 1.76 | 1.43 |
| behenoyl sphingomyelin (d18:1/22:0)* | 0.83 | 1.11 | 0.83 | 1.08 | 1.44 | 1.14 |
| sphingomyelin (d18:1/22:1, d18:2/22:0, d16:1/24:1)* | 0.79 | 1.09 | 0.89 | 1.26 | 1.72 | 1.43 |
| sphingomyelin (d18:1/20:0, d16:1/22:0)* | 0.82 | 1.00 | 0.86 | 1.16 | 1.46 | 1.20 |
| palmitoyl dihydrosphingomyelin (d18:0/16:0)* | 1.09 | 0.93 | 1.18 | 0.64 | 2.23 | 2.10 |
| sphingomyelin (d18:1/15:0, d16:1/17:0)* | 0.77 | 1.14 | 0.87 | 1.07 | 1.19 | 1.04 |
| sphingomyelin (d18:1/21:0, d17:1/22:0, d16:1/23:0)* | 0.79 | 0.96 | 0.79 | 1.44 | 1.70 | 1.52 |
| sphingomyelin (d18:2/23:0, d18:1/23:1, d17:1/24:1)* | 0.77 | 1.09 | 0.74 | 1.09 | 1.59 | 1.35 |
| sphingomyelin (d18:2/24:1, d18:1/24:2)* | 0.74 | 1.14 | 0.78 | 0.97 | 1.60 | 1.33 |
| tricosanoyl sphingomyelin (d18:1/23:0)* | 0.80 | 1.16 | 0.76 | 1.11 | 1.70 | 1.47 |
| sphingomyelin (d18:1/17:0, d17:1/18:0, d19:1/16:0) | 0.74 | 1.03 | 0.71 | 1.09 | 1.39 | 1.23 |
| glycosyl-N-stearoyl-sphingosine | 0.70 | 0.97 | 0.70 | 0.97 | 0.94 | 0.70 |
| glycosyl-N-palmitoyl-sphingosine | 0.62 | 0.91 | 0.90 | 0.99 | 1.10 | 0.91 |
| lactosyl-N-palmitoyl-sphingosine | 0.86 | 0.97 | 0.93 | 0.90 | 1.00 | 0.83 |
Table shows mean fold changes in fatty acid oxidation in THP-1 and NOMO-1 cells with NOX2 KD when compared to controls (transduced with control shRNA vector). Changes in sphingolipids were also compared in shTHP-1 and shNOMO-1 cells treated with DPI (100 nM) for 24 h or Mv4;11 cells treated with increasing GOX units/ml. Data shows relative values following normalization to protein concentration, log transformation and imputation of missing values with the minimum observed value for each compound (n = 4). Color boxes indicate ratios and p-values for each comparison: boxes shaded dark green indicate significant decreases and boxes shaded dark red indicate significant increases; statistical significance was performed using Welch’s two sample t-test (P < 0.05). Boxes shaded light green or red indicate decreases or increases, respectively and approaching significance (p < 0.1). * Indicates compounds that have not been officially confirmed based on a standard, but we are confident in its identity.