Figure 2.

Dose response plot of BF482 for MMP-9(A). The enzyme with and without inhibitor was pre-incubated for 30 min at room temperature and the reaction was started by adding McaPLGL(Dpa)AR-NH₂ (4 μM in assay). The reaction was allowed to proceed for 4 h at 37 °C and stopped by the addition of EDTA (10 mM end concentration). The relative fluorescence was determined with the Clariostar plate reader as described in the Experimental Section, where v_i and v₀ are the reaction rates in the presence and absence of BF482, respectively. Each point on the curve shows the mean ± sd (N = 5 for all points except for two points where N = 4). The regression coefficient r² is 0.97, with a determined IC₅₀ value of 38.8 ± 0.9 μM and a K_i value of 19.4 ± 0.4 μM.