. Author manuscript; available in PMC: 2021 Mar 25.

Published in final edited form as: Vet Pathol. 2018 May 22;55(5):622–633. doi: 10.1177/0300985818776054

Table 3.

Percentage of Cells With Immunolabeling for E-Cadherin, Vimentin, and SNAIL/SLUG⁺ in Primary Tumors (PT), Lymph Node Metastasis (LM), and Distant Metastasis (DM) of Canine Mammary Carcinoma.^a

	Percentage of Cells (Median, Range)			Pairwise Comparisons
Immunolabeling Pattern	PT (n = 20)	LM (n = 10)	DM (n = 16)	PT vs LM	PT vs DM
E-Cad⁺Vim⁻ membranous	13.0 (0.0–72.4)	7.4 (0.0–93.5)	10.1 (0.0–63.8)	P = .74	P = .23
E-Cad⁺Vim⁻ cytoplasmic	63.4 (2.8–94.6)	70.4 (0.0–89.2)	72.7 (20.2–100)	P = .77	P = .03^*
E-Cad⁺Vim⁻ total	84.6 (9.5–98.2)	83.3 (59.2–98.1)	86.8 (65.7–100)	P = .08	P = .04^*
E-Cad⁻Vim⁺	2.8 (0.0–62.6)	0.9 (0.0–11.4)	0.7 (0.0–8.9)	P = .01^**	P = .03^*
E-Cad⁺Vim⁺ (Cell count)	6.5 (0.0–28.4)	2.2 (0.3–11.6)	1.2 (0.0–11.5)	P = .02^*	P = .02^*
E-Cad⁺Vim⁺ (Area)	1.4 (0.003–4.1)	0.5 (0.1–2.5)	0.3 (0.0–1.5)	P = .06	P = .003^**
SNAIL/SLUG⁺	30.0 (0.0–95.0)	25.5 (10.0–55.0)	10.0 (4.0–75.0)	P = .95	P = .05^*

Cells with membranous and/or cytoplasmic E-cadherin labeling and concurrent vimentin labeling were counted as double positive, and were enumerated by 2 methods: cell counts and image analysis of an area. Pairwise comparisons were performed within the same animal between PT and LM and between PT and respective DM. Wilcoxon matched-paired tests.

P < .05

^**

P < .01.