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. 2021 Mar 25;21:321. doi: 10.1186/s12885-021-08056-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — (a and b) A TUNEL assay was used to detect SW480 cell apoptosis after treatment with RGD4C-p21Ras-scFv. More apoptotic cells were found in the RGD4C-scFv and RGD4C-linker-scFv groups than in the anti-p21Ras scFv group (green: apoptotic cell, blue: nucleus) (P < 0.05). (c) There was more green fluorescence were observed in the RGD4C-scFv and RGD4C-scFv+CQ groups of SW480, but other groups showed rarely green fluorescence signal. (d) More apoptotic cells were found in RGD4C-scFv+CQ compared with RGD4C-scFv group of SW480. (e and f) There were rarely green fluorescence observed with CACO-2 and CCD841 cells no matter RGD4C-scFv, RGD4C-scFv+CQ, RGD4C-EGFP and PBS groups. It implied that RGD4C-scFv did not induce CACO-2 and CCD841 cells apoptosis. Data of groups are presented as the mean ± S.D. *Significantly different from the control group (P < 0.05). **P < 0.01 vs controls