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Confocal photomicrographs from the ZI of Vgat-cre;L10-Egfp brain tissue (a) show EGFPVgat (b) in TH-ir neurons (c) but not immunoreactivity for dopamine β-hydroxylase (DBH; d). Filled arrowheads mark a representative sample of EGFPVgat TH-ir neurons (b, c) from the outlined area (a) that do not express DBH, as indicated by open arrowheads (d). Scale bars: 100 μm (a); 50 μm (b–d). V3, third ventricle.
